Nucleic acid isolation by organic extraction requires addition of phenol and guanidine isothiocyanate to independent the DNA, RNA, and proteins into distinct natural phases. If incomplete lysis is observed, increase the incubation time or amount of Proteinase K used for lysis. This optional built-in solitary-tube DNA Shearing streamlines the sample https://www.facebook.com/NewTechFind/posts/pfbid0eogsazG8w5FtRA6XuCL8PoznSQUnZSfmFmpyvCHeTgtpaue4wafpFntcSsYA1KP7l